To help expand substantiate that DNA binding is a prerequisite for the proteasomal degradation of CIC we fused a known SRY DNA binding domain name (DBD) construct to CIC wild type or CIC(R1515H) mutant resulting in DNA binding (Supplementary Determine?3S) and robust ubiquitylation of both the wild type and mutant CIC (Supplementary Physique?3S)
To help expand substantiate that DNA binding is a prerequisite for the proteasomal degradation of CIC we fused a known…