We also used a luciferase reporter assay in the embryonal carcinoma cell range P19 showing that Ascl1 activates transcription through the E1 element also to a lesser level through the E5 element which intact Ascl1 binding motifs are necessary for this activity (Statistics 1L and 1M)
We also used a luciferase reporter assay in the embryonal carcinoma cell range P19 showing that Ascl1 activates transcription through…