The suspension was centrifuged at 15000 rpm for 20 min at 37C (Sorvall RC5B, SS34) and the supernatant containing phages were collected and subjected to DEAE sepharose or CsCl-gradient purification
The suspension was centrifuged at 15000 rpm for 20 min at 37C (Sorvall RC5B, SS34) and the supernatant containing phages…